Anti-cancer Effect of Recombinant PI-Laterosporulin10 as a Novel Bacteriocin with Selective Cytotoxicity on Triple Negative Breast Cancer Cells

Anti-microbial peptides (AMPs) are important factors of the innate immune system which perform a comprehensive spectrum biological activities. The present study aims to examine expression, purification, and cytotoxic effects recombinant PI–laterosporulin10 (LS10) defensing-like peptide. PI is transmembrane peptide that helps increase penetration specificity targeting. PI–LS10 sequence was optimized fused Xa protease site achieve higher levels expression. Then Xa–PI–LS10 cloned into pET-32a (+) vector expressed in Escherichia coli BL21 (DE3). fusion protein with molecular weight about 25.3 kDa analyzed using SDS-PAGE. maximum expression observed 8 h post-induction. After enzymatic cleavage, purity > 300 µg/ ml as final yield. showed anti-cancer characteristics against breast cancer cell lines. MCF7 (non-triple negative) MDA-MB-231 (triple cells were treated different concentrations order evaluate their activity efficiency targeting triple negative cells. Our results suggest had significant effect comparison normal line. Triple (MDA-MB-231) proliferation inhibited 72 after treatment compare In conclusion, produced successfully its favorable line confirmed.